Fibronectin 1 anticorps (Biotin)

N° du produit ABIN298210

Cet anticorps Lapin Polyclonal détecte spécifiquement Fibronectin 1 dans WB, ELISA, IHC, IP et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache) et Singe.

Aperçu rapide pour Fibronectin 1 anticorps (Biotin) (ABIN298210)

Antigène: Fibronectin 1 (FN1)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin 1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Fibronectin 1 anticorps (Biotin)

Specificité: Other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross- reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible

Purification: Immunoaffinity purified

Immunogène: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single- band purity was assessed by SDS-PAGE.

Information d'application

Indications d'application: Approved: ELISA (1:4000 - 1:8000), IHC, IHC-Fr (1:50 - 1:200), IP, WB (1:10000)

Usage: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5000 to 1:10000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized, 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Détails sur Fibronectin 1

Antigène: Fibronectin 1 (FN1)

Autre désignation: FN1 / Fibronectin

Sujet: Name/Gene ID: FN1

Synonyms: FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

ID gène: 2335

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy